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The vector pCBC will be the cloning vector into which the gRNA modules were inserted independently. (B) Samples of the assembly of two-gRNA expression cassettes for dicots and monocots using the gRNA modules. Be aware: Every PCR fragment is flanked by two BsaI web pages (not demonstrated).

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? This menu's updates are based on your action. The data is barely saved locally (on the Laptop) and in no way transferred to us. You can click on these links to crystal clear your background or disable it.

The strains used for sequencing Evaluation are indicated with containers. (D) Alignment of sequences of mutated alleles determined from cloned PCR fragments from two agent T0 transgenic strains. Highlighting denotes the diploma of homology from the aligned fragments, and only aligned regions of curiosity are shown. The quantity of indels of a similar type is indicated.

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